Method of producing 2, 3-butanediol using recombinant yeast
AssigneeSnu R&Db Foundation
InventorsJin Ho Seo, Soo Jung Kim, Seung Oh Seo
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Abstract
Disclosed is a method of producing 2,3-butanediol using a recombinant yeast having a 2,3-butanediol biosynthesis pathway controlled in a metabolic engineering manner. The method enables production of 2,3-butanediol at high production efficiency from glucose or xylose using a recombinant yeast wherein enzymatic activity of pyruvate decarboxylase is inhibited and exotic genes associated with 2,3-butanediol biosynthesis are introduced.
Claims12
A recombinant Saccharomyces cerevisiae strain comprising genetic modifications such that functions of pyruvate decarboxylase are lost and an MTHI gene is modified such that the resultant MthI protein comprises a proline in place of an alanine at the 81st amino acid as set forth in SEQ ID NO:
- 17, and acetolactate synthase, acetolactate decarboxylase and butanediol dehydrogenase are expressed.
A recombinant Saccharomyces cerevisiae comprising genetic modifications such that xylose reductase, xylitol dehydrogenase and xylulose kinase are expressed, functions of pyruvate decarboxylase are lost, and acetolactate synthase, acetolactate decarboxylase and butanediol dehydrogenase are expressed and an MTHI gene is modified such that the resultant MthI protein comprises a proline in place of an alanine at the 81st amino add as set forth in SEQ ID NO:
- 17.
Dependencies are inferred from the claim text.