Aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method
AssigneeKorea University Research and Business Foundation
InventorsMan Bock Gu, Jee Woong Park, Tatavarty Rameshwar
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Abstract
Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases.
Claims15
A method for producing a nucleic acid aptamer without target immobilization comprising:
- reacting a single-stranded nucleic acid pool including a PCR primer region at both ends and any 30 to 50 bases at its center with a target material or a counter-target material and adding graphene;
- and
- separating a target specific nucleic acid aptamer by removing a target non-specific single-stranded nucleic acid bound to the graphene, or
- separating a target specific nucleic acid aptamer from the graphene by causing a conformational change by the target material on a target specific single-stranded nucleic acid bound to the graphene.
A nucleic acid aptamer having a sequence selected from the group consisting of the base sequences set forth in SEQ ID NOS:
- 4 to 18, wherein the nucleic acid aptamer is specifically bound to a Nampt protein.
A kit for detecting a Nampt protein comprising:
- a nucleic acid aptamer having a sequence selected from the group consisting of the base sequences set forth in SEQ ID NOS:
- 4 to 18, wherein the nucleic acid aptamer is capable of specifically binding to a Nampt protein.
A kit for diagnosing a Nampt related disease comprising:
- a nucleic acid aptamer having a sequence selected from the group consisting of the base sequences set forth in SEQ ID NOS:
- 4 to 18, wherein the nucleic acid aptamer is capable of specifically binding to a Nampt protein.
A composition for separating a Nampt protein comprising:
- a nucleic acid aptamer having a sequence selected from the group consisting of the base sequences set forth in SEQ ID NOS:
- 4 to 18, wherein the nucleic acid aptamer is capable of specifically binding to a Nampt protein.
Dependencies are inferred from the claim text.