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US 9,321,997 B2Granted
Tissue modeling in embryonic stem (ES) cell system
AssigneeAxiogenesis AG
InventorsEugen Kolossov, Jürgen Hescheler, Heribert Bohlen, Bernd Fleischmann, Wilhelm Röll, Andreas Ehlich, Jessica Königsmann
Life of this patent
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Abstract
Provided are embryonic stem (ES) cell-derived tissue modeling systems. In particular, systems for the de novo generation of tissue by parallel drug selection of cell types constituting the tissue of interest in one culture of differentiating ES cells is described as well as the use of such systems in transplantation and drug development.
Claims21
A method of modeling or producing a cardiac tissue comprising:
- (a) obtaining cardiomyocytes, wherein a first population of pluripotent cells is differentiated into said cardiomyocytes, wherein said differentiation includes forming embryoid bodies and isolating cardiomyocytes from said embryoid bodies;
- (b) obtaining fibroblasts, wherein a second population of pluripotent cells is differentiated into said fibroblasts;
- (c) co-culturing the cardiomyocytes obtained in step (a) with the fibroblasts obtained in step (b);
- (d) allowing integration and alignment of the cardiomyocytes and fibroblasts into a viable cardiac tissue;
- wherein the cardiomyocytes acquire longitudinal morphology upon integration and alignment with the fibroblasts;
- and
- wherein said viable cardiac tissue exhibits contractions for at least 2 weeks.
4 more elements
Dependencies are inferred from the claim text.