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US 9,321,994 B1Granted
Induction medium and methods for stem cell culture and therapy
AssigneeCommence Bio, Inc.
InventorAline Betancourt
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Abstract
Novel MSC stem-cell culture and therapy methods and culture medium compositions for the purpose of inducing, activating, or priming discrete uniform cell phenotypes to selectively promote or suppress inflammation and immunity, yielding polarized, primed, activated, or induced cells used in cell-based therapy.
Claims8
An induction medium for creating an immunologically polarized mesenchymal stem cell population from an unstimulated mesenchymal stem cell population, the induction medium comprising:
- a. a Toll-like receptor 4 (TLR4) ligand, at a concentration from about 1 nanogram per milliliter to about 100 micrograms per milliliter,
- b. erythropoietin, and
- c. 0.5-2.0% oxygen or a hypoxia mimetic,
- wherein the induction media creates the immunologically polarized mesenchymal stem cell population upon exposure of the induction media to the unstimulated mesenchymal stem cell population;
- and wherein the immunologically polarized mesenchymal stem cell population possesses pro-inflammatory characteristics marked by expression of pro-inflammatory mediators.
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Dependencies are inferred from the claim text.