Systems and methods for detecting the presence of a biological status using plot

A system for determining the presence of a biological entity in a sample, the system comprising:

• a measurement module capable of detecting values associated with at least three genetic elements in the sample;
• a memory storing the detected values from the measurement module;
• a computer readable medium containing computer readable code having instructions for executing a call algorithm, wherein the call algorithm uses as inputs the detected and measured values of the at least three genetic elements and combines them to form a vector, which is compared to a boundary function on a 2-dimensional Y-plot comprising an origin, and if the vector is within the boundary function, the biological entity is present and if the vector is not within the boundary function, the biological entity is not present;
• and
• a processor to execute the computer readable code on the computer readable medium in order to determine the presence of the biological entity in the sample, and wherein vectors associated with the biological entity are generally closer to the origin than vectors that are not associated with the biological entity.

A method for creating a model that can be used to determine the presence of a biological entity in an unknown sample, the method comprising:

• detecting the presence and values of at least three genetic elements in known samples;
• executing a call algorithm on a digital computer for each sample in the known samples, wherein the call algorithm creates vectors and uses as inputs for each vector the detected values of the at least three genetic elements, wherein some vectors are associated with the biological entity and some vectors are not associated with the biological entity;
• plotting the vectors on a 2-dimensional Y-plot comprising an origin;
• and
• creating a boundary function separating the vectors that are associated with the biological entities and the vectors that are not associated with the biological entity, and wherein vectors associated with the biological entity are generally closer to the origin than vectors that are not associated with the biological entity.