Lid separation device and methods

A separation device for separating a supernatant from a solid phase, comprising

• a multiwell plate for holding one or more liquids comprising one or more analytes and a solid phase to which said analyte can be bound, said multiwell plate comprising
• at least two purification chambers holding said liquid and said solid phase, wherein one or more of said analytes are bound to said solid phase within said purification chambers, said purification chambers not fluidically connected to each other or any other chamber in an opposite direction of said liquid's flow direction,
• at least one waste chamber to which the liquid can be transferred following binding of the analyte to the solid phase, said at least one waste chamber being fluidically connected only to one of said at least two purification chambers or to more than one of said at least two purification chambers without being further fluidically connected to any other chamber in said liquid's flow direction, and
• a lid covering said multiwell plate,
• wherein the interface between said multiwell plate and said lid is a gap in a side wall of the purification chambers, or a gap or a channel present on the lid, said gap in the side wall, or said gap or said channel present on the lid, connecting the purification chambers with the waste chamber to allow the liquid to transfer from the purification chambers holding the solid phase to said waste chamber when exposed to centrifugal force after said one or more analyte is bound to said solid phase, while the solid phase remains in said purification chambers, wherein said side wall of the purification chambers comprises an inclined surface toward the waste chamber.