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US 9,316,652 B2Granted
Method for diagnosing Lyme disease using a cellular immunological test
AssigneeStichting Katholieke Universiteit
InventorsLeonardus Antonius Bernardus Joosten, Mihai Gheorghe Netea, Johannes Willem Maarten van der Meer, Bart Julian Kullberg
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Abstract
The present invention relates to a method for diagnosing Lyme disease in a subject, the method comprising the steps of: (a) obtaining a sample from said subject, (b) contacting said sample with a source of Borrelia antigens and (c) determining the expression level of a pro-inflammatory cytokine in said sample at the end of step (b).
Claims31
A method for diagnosing Lyme disease in a subject, the method comprising the steps of:
- (a) contacting a sample obtained from said subject with a source of Borellia antigen from a species of Borellia selected from the group consisting of B. burgdorferi, B. garinii, B. afzelii and combinations thereof, wherein said sample comprises peripheral blood mononuclear cells (PBMC);
- (b) determining a protein expression level of IL-1β in said sample at the end of step (a);
- and
- (c) diagnosing Lyme disease in said subject when a detectable expression level or an increase of the expression level of IL-1β is determined in step (b).
1 more element
A method for diagnosing Lyme disease in a subject, the method comprising the steps of:
- (a) contacting a sample obtained from said subject with a source of Borellia antigen from a species of Borellia selected from the group consisting of B. burgdorferi, B. garinii, B. afzelii and combinations thereof, wherein said sample comprises peripheral blood mononuclear cells (PBMC);
- (b) determining protein expression of IL-1β in said sample at the end of step (a);
- and
- (c) diagnosing Lyme disease in said subject when detectable expression of IL-1β is determined in step (b).
1 more element
Dependencies are inferred from the claim text.