Devices and methods for detection of Panton-Valentine Leukocidin (PVL)

A method of detecting a biomolecule in a sample, comprising:

• a) contacting a sample with an antibody, or a functional binding fragment thereof, that specifically binds an expression product of one or more genes selected from lukS-PV, lukF-PV, lukM, lukF-P83, mecA, and spa, to form a conjugate;
• and
• b) detecting the conjugate of (a), thereby detecting the toxin,
• wherein the antibody, or the functional binding fragment thereof, comprises an amino acid sequence as set forth in SEQ ID NOs:
• 2, 4, 6, 8, 10 or 12.