Biosensor for detection of subclinical ketosis
AssigneeUniversity of Guelph
InventorsSuresh Neethirajan, Xuan Weng, Longyan Chen
Life of this patent
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Abstract
A method of detecting subclinical ketosis in a dairy animal comprises the steps of: obtaining a sample from the dairy animal, the sample containing one of a serum sample or a milk sample; incubating a mixture of the sample and a reactant containing NAD+-functionalized quantum dots to produce an incubated mixture; irradiating the incubated mixture with optical radiation of a first wavelength so as to emit fluorescent radiation of a second wavelength from the incubated mixture; irradiating a photodetector with the fluorescent radiation so as to generate an electrical signal; and performing data acquisition using the photodetector electrical signal to determine a BHBA concentration for the sample.
Claims13
A method of detecting subclinical ketosis in a dairy animal, said method comprising the steps of:
- obtaining a sample from the dairy animal, said sample containing one of a serum sample or a milk sample;
- incubating a mixture of said sample and a reactant containing NAD+-functionalized quantum dots to produce an incubated mixture;
- irradiating said incubated mixture with optical radiation of a first wavelength so as to emit fluorescent radiation of a second wavelength from said incubated mixture;
- irradiating a photodetector with said fluorescent radiation so as to generate an electrical signal;
- and
- performing data acquisition using said photodetector electrical signal to determine a β-hydroxybutyrate (BHBA) concentration for the sample.
A method of detecting subclinical ketosis using a sample from a dairy animal, said method comprising the steps of:
- preparing a reactant containing NAD+-functionalized quantum dots by modifying a plurality of carboxyl group capped quantum dot nanoparticles with 3-aminophenyl boronic acid (APBA) with a carbodiimide reaction;
- incubating said reactant with the sample to produce an incubated mixture;
- irradiating said incubated mixture with 470 nm optical radiation;
- irradiating a photodetector with fluorescent radiation emitted from said incubated mixture so as to generate an electrical signal;
- and
- performing data acquisition on said electrical signal to determine a BHBA concentration for the sample.
A biosensor system suitable for detecting subclinical ketosis (SCK) in a subject animal from a sample of blood serum or milk obtained from the subject animal, said system comprising:
- a reactant containing a plurality of nicotinamide adenine dinucleotide (NAD+)-functionalized quantum dots;
- a microfluidic chip having a sensing well for receiving an assay mixture of the sample and said reactant;
- an optical sensor assembly having an optical radiation source for inducing fluorescent radiation in said assay mixture, and an optical detector for producing an electrical signal in response to said fluorescent radiation;
- and
- at least one of a microcontroller board, a computer, and a mobile communication device for receiving said electrical signal and performing data acquisition to determine the amount of β-hydroxybutyrate (BHBA) in the sample.
Dependencies are inferred from the claim text.