Bioassay method for detecting physiologically active substance
AssigneeOtsuka Pharmaceutical Co., Ltd.
InventorsNaohiro Araki, Mitsuru Iida, Kiyotaka Machida
Life of this patent
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Abstract
Provided are a procedurally simple and safe method and kit for measuring the concentration in a biological sample of a physiologically active substance which binds to a receptor that causes a change in intracellular cAMP concentration. By providing a composition including genetically-modified cells which show co-expression of a receptor that causes a change in intracellular cAMP concentration, a cyclic nucleotide-responsive calcium channel, and aequorin luminescent protein, it is possible to measure how much of a physiologically active substance which binds to said receptor is included in a biological sample.
Claims13
A transformed cell, wherein the cell expresses Gs-coupled GPCR, wherein the GS-coupled GPCR is a GLP-1 receptor or a vasopressin V2 receptor,
- a modified CNG calcium channel having the amino acid sequence represented by SEQ ID NO:
- 2, and
- a modified apoaequorina having the amino acid sequence represented by SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.