Sample detection apparatus and detection method

A sample detection method comprising:

• using a sample detection apparatus including an insulating partition configured to divide a first region and a second region, a pore formed in the partition and configured to make the first region and the second region communicate, a first electrode arranged in the first region, and a second electrode arranged in the second region;
• introducing a reagent containing a capture substance to be specifically bound to a target to be detected and a tag particle bound to the capture substance into the first region together with a sample, and filling the second region with an electrolyte solution;
• applying electrical current between the first electrode and the second electrode through the pore, and observing a change in a conductive state between the first electrode and the second electrode when the target and the tag particle to be detected in the first region passes through the pore;
• and
• judging presence/absence of the target to be detected in the sample based on the measured change in the conductive state,
• wherein:
• the reagent contains a first capture substance to be specifically bound to a first portion of the target to be detected, a first tag particle bound to the first capture substance, a second capture substance to be specifically bound to a second portion of the target to be detected, and a second tag particle bound to the second capture substance, and
• in the judging the presence/absence of the target to be detected, it is judged that the target to be detected exists in the sample when the change in the conductive state caused by passage of the first tag particle and the change in the conductive state caused by passage of the second tag particle are detected continuously or in a combinational manner.