US 9,315,870 B2 · Method for detecting methylation of colorectal cancer specific methylation marker gene for colorectal cancer diagnosis

Abstract

The present invention relates to a method for detecting methylation of the bowel-cancer-specific methylation marker GPM6A (NM_201591, glycoprotein M6A) gene in order to diagnose bowel cancer, and more specifically relates to a method for providing information for diagnosing bowel cancer by detecting the methylation of a bowel-cancer-specific marker gene that is specifically methylated in bowel cancer cells. The method for detecting methylation and a diagnostic composition, kit and nucleic-acid chip according to the present invention can be used to advantage in diagnosing bowel cancer more accurately and quickly than by normal methods as they permit bowel cancer to be diagnosed at the initial genetic transformation step and so allow early diagnosis.

Claims10

Dependent claims

A method for detecting CpG methylation of GPM6A (glycoprotein M6A) gene, the method comprising the steps of:

(a) isolating genomic DNA from a clinical sample of a human subject selected from the group consisting of colorectal tissue, feces, urine, serum, and blood plasma;
(b) treating the genomic DNA or a fragment thereof with bisulfite, wherein the genomic DNA or fragment thereof is treated with bisulfite in such a way that cytosine is converted into a base that is different in its base-pairing behavior in the DNA duplex;
and
(c) determining hypermethylation of the CpG of the promoter or the CpG of the first intron of GPM6A gene in the genomic DNA or fragment thereof treated with bisulfite according to step (b) with primers selected from the group consisting of SEQ ID NOS:
16 to 19.

2 more elements

Dependencies are inferred from the claim text.

Method for detecting methylation of colorectal cancer specific methylation marker gene for colorectal cancer diagnosis

Life of this patent

Method for detecting methylation of colorectal cancer specific methylation marker gene for colorectal cancer diagnosis

Life of this patent

Abstract

Claims10