Conjugates of nucleotides and method for the application thereof
AssigneeGenovoxx GmbH
InventorsClaus Becker, Dmitry Cherkasov
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Abstract
The invention relates to a novel method for enzymatically marking nucleic acid chains (target sequences) by using nucleotide conjugates. Said nucleotide conjugates are capable of binding specifically to the target sequence under reaction conditions and of being incorporated in the complementary growing strand by means of a polymerase. The nucleic acid chains marked with such conjugates can be bound to the solid phase. The marking can be carried out in parallel with the enzymatic amplification of target sequences.
Claims51
A method for enzymatic synthesis of one or more nucleic acid chains, comprising the step of enzymatic incorporation of nucleotides into the complementary strand of at least one target sequence or equivalents thereof, wherein at least one of the nucleotides is a nucleotide-conjugate having the structure:
- (Nuc-linker)n- marker
- wherein:
- Nuc is a nucleotide (nuc-component);
- Linker is a linker component, wherein the linker links the nuc-component to a macromolecular marker component;
- Marker is a macromolecular marker component which comprises at least one target domain having an oligonucleotide sequence complementary to a portion of the target sequence or equivalents thereof;
- and
- n is a positive integer from 1 to 1000;
- and
- wherein binding of the at least one target domain of the nucleotide-conjugate to the complementary portion of the target sequence facilitates selective incorporation of the said nucleotide-conjugate into the synthesized strand of the one or more nucleic acid chains.
Dependencies are inferred from the claim text.