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US 9,315,836 B2Granted
Fatty acid desaturase used in the biosynthesis of long chain omega-3 polyunsaturated fatty acids
AssigneeUniversity of Jiangnan
InventorsHaiqin Chen, Zhennan Gu, Hao Zhang, Wei Chen, Yuanda Song, Fengwei Tian, Jianxin Zhao, Yongquan Chen
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Abstract
The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially ω3 desaturases (FADS15). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases.
Claims4
A process for producing a purified omega-3 desaturase, comprising:
- (a) constructing a pPinkα-HC vector containing cDNA molecule having the nucleotide sequence shown as SEQ ID NO:1;
- (b) transforming a suitable host cell with said vector of Step (a), wherein said host cell is a yeast cell;
- (c) culturing said host cell under conditions which allow the expression of said omega-3 desaturase by said host cell;
- and
- (d) isolating said omega-3 desaturase from the culture media of Step (c);
- wherein said purified omega-3 desaturase is produced.
3 more elements
Dependencies are inferred from the claim text.