Lysophospholipid acyltransferase

A cDNA or recombinant vector comprising a nucleic acid of any one of (a)-(e) below:

• (a) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence with deletion, substitution or addition of one to 50 amino acids in the amino acid sequence shown in SEQ ID NO:
• 2, and having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector;
• (b) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a nucleotide sequence full length complement to the nucleotide sequence consisting of SEQ ID NO:
• 1 and that comprises a nucleotide sequence encoding a protein having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector;
• (c) a nucleic acid that comprises a nucleotide sequence sharing an identity of 90% or more with the nucleotide sequence consisting of SEQ ID NO:
• 1 and encoding a protein having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector;
• (d) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence sharing an identity of 90% or more with the amino acid sequence consisting of SEQ ID NO:
• 2 and having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector;
• and
• (e) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a nucleotide sequence full length complement to a nucleotide sequence encoding a protein consisting of the amino acid sequence shown in SEQ ID NO:
• 2 and that comprises a nucleotide sequence encoding a protein having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector.

A cDNA or recombinant vector comprising a nucleic acid of any one of (a)-(d) below:

• (a) a nucleic acid that comprises the nucleotide sequence shown in SEQ ID NO:
• 1;
• (b) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of the amino acid sequence shown in SEQ ID NO:
• 2;
• (c) a nucleic acid that comprises the nucleotide sequence shown in SEQ ID NO:
• 4;
• and
• (d) a nucleic acid that comprises the nucleotide sequence shown in SEQ ID NO:
• 5.

A cDNA or recombinant vector comprising a nucleic acid of any one of (a)-(e) below:

• (a) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence with deletion, substitution or addition of one to 50 amino acids in the amino acid sequence shown in SEQ ID NO:
• 2, and involved in the conversion from 18:3(n-6)-PL to 18:3(n-6)-CoA or conversion from DGLA-CoA to DGLA-PL;
• (b) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a full length complement to the nucleotide sequence consisting of SEQ ID NO:
• 1 and that comprises a nucleotide sequence encoding a protein involved in the conversion from 18:3(n-6)-PL to 18:3(n-6)-CoA or conversion from DGLA-CoA to DGLA-PL;
• (c) a nucleic acid that comprises a nucleotide sequence sharing an identity of 90% or more with the nucleotide sequence consisting of SEQ ID NO:
• 1 and encoding a protein involved in the conversion from 18:3(n-6)-PL to 18:3(n-6)-CoA or conversion from DGLA-CoA to DGLA-PL;
• (d) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence sharing an identity of 90% or more with the amino acid sequence consisting of SEQ ID NO:
• 2 and involved in the conversion from 18:3(n-6)-PL to 18:3(n-6)-CoA or conversion from DGLA-CoA to DGLA-PL;
• and
• (e) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a full length complement to a nucleotide sequence encoding a protein consisting of the amino acid sequence shown in SEQ ID NO:
• 2 and that comprises a nucleotide sequence encoding a protein involved in the conversion from 18:3(n-6)-PL to 18:3(n-6)-CoA or conversion from DGLA-CoA to DGLA-PL.