Methods for intracellular delivery of nucleic acids
AssigneeUniversite Paris Descartes
InventorsFrancine Behar-Cohen, Elodie Touchard
Life of this patent
Estimating the term…
Abstract
A method for intracellular delivery of isolated naked nucleic acids into a biological tissue or organ, that includes (a) contacting the tissue or organ with an efficient amount of at least one active corticosteroid, and (b) contacting the tissue or organ treated in step b with an efficient amount of isolated naked nucleic acids, wherein step (a) is carried out for a period of time ranging from at least five minutes to at most two hours and being immediately followed by step (b).
Claims6
A method for intracellular delivery of at least one isolated naked nucleic acid into an ophthalmic tissue comprising a non-competent cell, said method comprising at least the steps of:
- a/ contacting said ophthalmic tissue with an efficient amount of at least one active corticosteroid for a period of time ranging from at least five minutes to at most two hours;
- and, immediately following step a/,
- b/ contacting said ophthalmic tissue treated in step a/ with an efficient amount of at least one isolated naked nucleic acid by injection into ciliary muscle;
- wherein:
- said at least one active corticosteroid is selected from the group consisting of triamcinolone, anecortave, derivatives thereof, and mixture thereof, and
- said method does not include an electrotransfer step.
As method for promoting intracellular delivery of an isolated naked nucleic acid in an individual in need thereof, the method comprising:
- administering to said individual a pharmaceutical composition comprising at least one corticosteroid as an active agent at least 5 minutes to at most 2 hours before administering to said individual said isolated naked nucleic acid, said isolated naked nucleic acid being administered by injection in a ciliary muscle in an ophthalmic tissue comprising a non-competent cell,
- wherein;
- said method does not include an electrotransfer step, and
- said at least one active corticosteroid is selected from the group consisting of triamcinolone, anecortave, derivatives thereof, and mixture thereof.
Dependencies are inferred from the claim text.