US 9,315,828 B2 · Prompt nucleic acid delivery carrier composition

Abstract

An object of the present invention is to provide a carrier composition for nucleic acid delivery, which can efficiently deliver a nucleic acid into cells when a nucleic acid such as siRNA is administered to animal-derived cells or animals, and also has low toxicity and high safety, and a composition for nucleic acid delivery containing the carrier composition and nucleic acid. A carrier for nucleic acid delivery is prepared by using (A) a diacylphosphatidylcholine, (B) at least one member selected from the group consisting of cholesterol and derivatives thereof, and (C) an aliphatic primary amine. Also, a composition for nucleic acid delivery is prepared by mixing the carrier for nucleic acid delivery with a nucleic acid.

Claims7

Dependent claims

A composition for delivery of siRNA comprising an siRNA, and a carrier composition for delivery of siRNA, consisting essentially of (A) at least one member selected from the group consisting of dimyristoylphosphatidycholine and distearoylphosphatidylcholine , (B) cholesterol , and (C) an aliphatic primary amine, wherein the molar ratio of component (A):

component (B):
component (C) is 6-9:1-4:1, wherein the total amount of components (A) to (C) contained in the composition for delivery of siRNA is from 30 to 90% by weight, based on the total amount of the composition.

Dependencies are inferred from the claim text.

Facts

Application

12/532,949Filed 26 Mar 2008

Priority

JP 2007-7994426 Mar 2007

Published

US 2010/0063131 A111 Mar 2010

Granted

19 Apr 2016

Term ends

Not estimatedThe source records don’t support an estimate.

Classification13 codes

C12N 15/87
Recombinant DNA-technology: introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
A61K 9/127
Dispersions; Emulsions: synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
A61K 31/7088
Carbohydrates; Sugars; Derivatives thereof: compounds having three or more nucleosides or nucleotides
A61K 31/713
Compounds having three or more nucleosides or nucleotides: double-stranded nucleic acids or oligonucleotides

Prompt nucleic acid delivery carrier composition

Life of this patent

Prompt nucleic acid delivery carrier composition

Life of this patent

Abstract

Claims7