Nucleic acids containing a synthetic codon-optimized Sin Nombre virus full-length M gene
AssigneeThe United States of America as rep. by the Sec'y of the Army, for U.S. Army Medical Research Institute of Infectious Diseases
InventorJay Hooper
Life of this patent
Estimating the term…
Abstract
The invention contemplates a new synthetic, codon-optimized Sin Nombre virus (SNV) full-length M gene open reading frame (ORF) that encodes a unique consensus amino acid sequence. The SNV ORF was cloned into a plasmid to form the first stable recombinant SNV full-length M gene that elicits neutralizing antibodies. The gene can be engineered into a vaccine system, and is useful to protect mammals against infection with Sin Nombre virus.
Claims8
An isolated nucleic acid sequence set forth in SEQ ID NO:1.
A recombinant DNA construct comprising:
- (i) a vector, and
- (ii) the DNA fragment comprising the nucleic acid sequence set forth in SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3, operably linked to a promoter sequence.
A DNA cassette comprising either SEQ ID NO:2 or SEQ ID NO:3, linked to a promoter operable in a eukaryotic expression system.
Dependencies are inferred from the claim text.