Plant expression constructs and methods of utilizing same
AssigneesYissum Research Development Company of the Hebrew University of Jerusalem, Morflora Israel Ltd.
InventorsIlan Sela, Rita Mozes-Koch, Yuval Peretz, Herve Huet
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Abstract
A Geminivirus based expression construct being capable of systemic symptomeless spread in a plant host is provided as well as methods of utilizing same for plant gene expression, gene silencing and plant protection.
Claims15
A tomato yellow leaf curl virus (TYLCV) Geminivirus based expression construct comprising a polynucleotide sequence encoding:
- (i) a TYLCV coat protein (CP) having a deletion in nucleotides encoding an N-terminal 100 amino acids of said CP, wherein said deletion results in a deletion in the C terminus of the TYLCV V2 protein;
- and
- (ii) a TYLCV replicase having an insertion, wherein said insertion results in a reduced capability of rolling circle, single stranded DNA replication compared to an unmodified TYLCV replicase, and further wherein said insertion of said TYLCV replicase results in an insertion in the open reading frame of the C4 protein of said TYLCV.
A method of expressing a molecule of interest in a plant cell comprising introducing into the plant cell a TYLCV Geminivirus based expression construct comprising a polynucleotide sequence encoding:
- (i) a TYLCV coat protein (CP) having a deletion in nucleotides encoding an N-terminal 100 amino acids of said CP, wherein said deletion results in a deletion in the C terminus of the TYLCV V2 protein:
- and
- (ii) a TYLCV replicase having an insertion, wherein said insertion results in a reduced capability of rolling circle, single stranded DNA replication compared to an unmodified TYLCV replicase, and further wherein said insertion o said TYLCV replicase results in an insertion in the open reading frame of the C4 protein of said TYLCV;
- and
- (iii) a heterologous polynucleotide sequence encoding the molecule of interest.
Dependencies are inferred from the claim text.