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US 9,315,801 B2Granted
Method for separating and detecting an analyte
AssigneeRoche Molecular Systems, Inc.
InventorsRenato Belz, Andreas Gisler, Robert Huesler, Rolf Knobel, Christian Thalmann
Life of this patent
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Abstract
A method for separating and analyzing an analyte is provided which comprises, in a first position, transferring a liquid sample to a multiwell plate with a pipette tip, replacing the tips in the tip rack in the same position, and re-using the pipette tips for aspirating and dispensing liquid.
Claims10
A method for isolating an analyte that may be present in a fluid sample, the method comprising the automated steps of:
- a1) engaging pipette tips which are held in a rack in a first position with a first process head;
- a2) transferring said fluid sample from a sample vessel to a processing vessel with said pipette tips engaged to said first process head;
- a3) placing said pipette tips in said rack and disengaging said pipette tips from said process head;
- a4) transporting said rack comprising said pipette tips and said processing vessel to a second position;
- and
- a5) engaging said pipette tips which are held in said rack with a second process head in said second position;
- b) combining together a solid support material and said fluid sample in a well of said processing vessel for a period of time and under conditions sufficient to permit said analyte to be immobilized on the solid support material;
- c) isolating the solid support material from other material present in the fluid sample in a separation station;
- and
- d) purifying the analyte in the separation station by separating the fluid sample from the solid support material and washing the solid support materials one or more times with a wash buffer;
- wherein said pipette tips used in step a1) to a5) is re-used after steps a1) to a5).
8 more elements
A method for isolating an analyte that may be present in a fluid sample, the method comprising the automated steps of;
- a) transferring said fluid sample from a sample vessel to a processing vessel with a pipette tip;
- b) combining together a solid support material and said fluid sample in a well of said processing vessel for a period of time and under conditions sufficient to permit said analyte to be immobilized on the solid support material;
- c) isolating the solid support material from other material present in the fluid sample in a separation station;
- and
- d) purifying the analyte in the separation station by separating the fluid sample from the solid support material and washing the solid support materials one or more times with a wash buffer;
- wherein said pipette tip used in step a) is re-used after step a), and wherein said rack comprising said pipette tips and said processing vessel is transported from a separate first cell of an analytical instrument to a second position in a separate second cell of said analytical instrument.
3 more elements
Dependencies are inferred from the claim text.