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US 9,315,800 B2Granted
Method for isolating nucleic acids and composition used therefor
AssigneeIndustrial Technology Research Institute
InventorsYi-Chang Chen, Jane S-C Tsai
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Abstract
The disclosure provides a composition and method for isolating nucleic acids, in which the composition includes at least one halocarbon, at least one salt and at least one surfactant. Mixing the composition and a biosample to form a homogenized solution, nucleic acids in the solution can be easily isolated with a simple treatment and good yield.
Claims20
A composition for isolating nucleic acids, comprising
- at least one perfluorocarbon,
- at least one salt, and
- at least one surfactant,
- wherein the composition contains 1˜70% by weight of the perfluorocarbon based on the total weight of the composition, and
- wherein the perfluorocarbon comprises perfluoropentane, perfluorohexane, perfluoroheptane or perfluorooctane.
2 more elements
Dependencies are inferred from the claim text.