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US 9,315,793 B2Granted
Enzymes that synthesize zingiberene
AssigneeBoard of Trustees of Michigan State University
InventorsCornelius Barry, Eliana Gonzales-Vigil
Life of this patent
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Abstract
The invention relates to nucleic acids encoding a zingiberene synthase that enables host cells and plants to make zingiberene that is useful in fragrances and for repelling or killing insects. The invention also relates to isolated zingiberene synthases and to methods for making zingiberenes.
Claims9
A method comprising:
- a) culturing a host cell to express a zingiberene synthase, wherein the host cell comprises an isolated nucleic acid encoding the zingiberene synthase operably linked to a heterologous promoter, and wherein the zingiberene synthase has at least 98% sequence identity to amino acid sequence SEQ ID NO:
- 2, 4, 8, 11, 12, 14, or 18;
- and
- b) providing the host cell with a substrate for the zingiberene synthase.
1 more element
A seed or plant part comprising a heterologous DNA encoding an amino acid sequence selected from SEQ ID NO:
- 2, 4, 8, 11, 12, 14 or 18.
A seed or plant part comprising a DNA encoding an amino acid sequence with at least 98% sequence identity to any of SEQ ID NOs:
- 2, 4, 8, 11, 12, 14, or 18, wherein the DNA is operably linked to a heterologous promoter.
A method comprising:
- contacting an isolated zingiberene synthase with a substrate for the zingiberene synthase, where the isolated zingiberene synthase has at least 98% sequence identity to any of SEQ ID NOs:
- 2, 4, 8, 11, 12, 14, or 18, and a conservative or non-conservative substitution.
Dependencies are inferred from the claim text.