Nitrilases, nucleic acids encoding them and methods for making and using them
AssigneeBASF Enzymes LLC
InventorsKelvin Wong, Jay M. Short, Mark J. Burk, Grace DeSantis, Robert Farwell, Kelly Chatman
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Abstract
The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
Claims8
An isolated, synthetic, or recombinant variant polypeptide having nitrilase activity, wherein the variant polypeptide is at least 90-99% identical to the full length amino acid sequence of SEQ ID NO:
- 238;
- and comprises at least one mutation at an amino acid position selected from the group consisting of:
- 55, 60, 111, 190, 191, 199, or any combination thereof.
An isolated, synthetic, or recombinant variant polypeptide having a nitrilase activity wherein the variant polypeptide is at least 90%-99% identical to the full length of SEQ ID NO:
- 238 and has at least one mutation selected from A55G, A55K, 160E, N111S, A190T, A1905, A190H, F191L, F191T, F191M, F191V, M199E, M199L, or any combination thereof.
Dependencies are inferred from the claim text.