Metalloproteases from alicyclobacillus
AssigneeNovozymes A/S
InventorsAstrid Benie, Peter Rahbek Oestergaard, Morten Gjermansen
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Abstract
The present invention relates to new metalloproteases derived from Alicyclobacillus and the use thereof in cleaning processes, such as laundry and dish wash, and in particular to the use in low temperature wash and in removal of egg stains. The invention also relates to detergent compositions and cleaning compositions comprising Alicyclobacillus sp. metalloproteases.
Claims20
A method of producing a polypeptide having protease activity, comprising:
- (a) cultivating a recombinant cell comprising a polynucleotide encoding the polypeptide under conditions conducive for production of the polypeptide, wherein the polypeptide has at least 80% sequence identity to the sequence of amino acids 214-511 of SEQ ID NO:
- 2 or the sequence of amino acids 214-517 of SEQ ID NO:
- 4 and the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide;
- and
- (b) recovering the polypeptide.
A nucleic acid construct or expression vector comprising a gene encoding a protein operably linked to a polynucleotide encoding a signal peptide of amino acids 1 to 25 of SEQ ID NO:
- 2 or amino acids 1 to 25 of SEQ ID NO:
- 4, wherein the gene is foreign to the polynucleotide encoding the signal peptide.
Dependencies are inferred from the claim text.