Antibody conjugates
AssigneeVentana Medical Systems, Inc.
InventorsChristopher Bieniarz, Jennifer Wong, Mark Lefever, Jerome W. Kosmeder, II, Julia Ashworth-Sharpe, Casey A. Kernag
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Abstract
Antibody/signal-generating moiety conjugates are disclosed that include an antibody covalently linked to a signal-generating moiety through a heterobifunctional polyalkyleneglycol linker. The disclosed conjugates show exceptional signal-generation in immunohistochemical and in situ hybridization assays on tissue sections and cytology samples. In one embodiment, enzyme-metallographic detection of nucleic acid sequences with hapten-labeled probes can be accomplished using the disclosed conjugates as a primary antibody without amplification.
Claims12
An antibody-PEG linker-enzyme signal-generating moiety conjugate, comprising:
- an antibody;
- a plurality of enzyme signal-generating moieties;
- and
- a plurality of monodisperse, heterobifunctional PEG linkers, wherein each enzyme signal-generating moiety is linked to the antibody through at least one of the plurality of monodisperse, heterobifunctional PEG linker to provide the antibody-PEG linker-enzyme signal-generating moiety conjugate having the formula
- wherein Ab is the antibody, SM is the enzyme signal-generating moiety, n=4 to 12 and s=3 to 10.
Dependencies are inferred from the claim text.