Pyripyropene a biosynthetic gene
AssigneeMeiji Seika Pharma Co., Ltd.
InventorsHiroyuki Anzai, Kentaro Yamamoto, Mariko Tsuchida, Kazuhiko Oyama, Masaaki Mitomi
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Abstract
An isolated novel polynucleotide comprising a nucleotide sequence encoding at least one polypeptide involved in biosynthesis of pyripyropene A, a recombinant vector comprising the polynucleotide and a transformant comprising the polynucleotide are disclosed. By the present invention, a pyripyropene A biosynthetic gene useful for production of a novel pyripyropene analog, improvement of productivity of a pyripyropene A-producing bacterium, production of an insecticidal agent for microorganisms, creation of a plant resistant to insect pests or the like are provided.
Claims8
An isolated polynucleotide comprising a selection marker, and
- (a) a polynucleotide having a polynucleotide sequence encoding at least one amino acid sequence selected from SEQ ID NOs:
- 267 to 274 or an amino acid sequence selected from SEQ ID Nos:
- 267 to 274 having 1 to 40 substituted, deleted, added or inserted residues and having the same enzymatic activity as SEQ ID NOs:
- 267 to 274, respectively, or
- (b) a polynucleotide having a nucleotide sequence which is capable of hybridizing with the nucleotide sequence of SEQ ID NO:
- 266 under stringent conditions comprising washing in a solution comprising 30 mM trisodium citrate, 300 mM sodium chloride and 0.5% sodium dodecyl sulfate at 60° C. for 20 minutes and having the same enzymatic activity as SEQ ID NO:
- 266,
- wherein the polynucleotide of (a) or (b) is a cDNA.
An isolated cDNA, which has at least one nucleotide sequence selected from the nucleotide sequence of any of (1) or (2) below:
- (1) a nucleotide sequence of any of (a) to (h) below:
- (a) a nucleotide sequence from positions 3342 to 5158 of the nucleotide sequence shown in SEQ ID NO:
- 266,
- (b) a nucleotide sequence from positions 5382 to 12777 of the nucleotide sequence shown in SEQ ID NO:
- 266,
- (c) a nucleotide sequence from positions 13266 to 15144 of the nucleotide sequence shown in SEQ ID NO:
- 266,
- (d) a nucleotide sequence from positions 16220 to 18018 of the nucleotide sequence shown in SEQ ID NO:
- 266,
- (e) a nucleotide sequence from positions 18506 to 19296 of the nucleotide sequence shown in SEQ ID NO:
- 266,
- (f) a nucleotide sequence from positions 19779 to 21389 of the nucleotide sequence shown in SEQ ID NO:
- 266,
- (g) a nucleotide sequence from positions 21793 to 22877 of the nucleotide sequence shown in SEQ ID NO:
- 266,
- (h) a nucleotide sequence from positions 23205 to 24773 of the nucleotide sequence shown in SEQ ID NO:266;
- (2) a nucleotide sequence which is capable of hybridizing with the nucleotide sequence of (1) under stringent conditions comprising washing in a solution comprising 30 mM trisodium citrate, 300 mM sodium chloride and 0.5% sodium dodecyl sulfate at 60° C. for 20 minutes,
- wherein the nucleotide sequences corresponding to (1) (a) to (h) and (2) encode the same enzymatic activity as the sequences of SEQ IDS Nos:
- 267 to 274, respectively.
Dependencies are inferred from the claim text.