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US 9,315,779 B2Granted
Reprogramming cells
AssigneeThe Scripps Research Institute
InventorsSaiyong Zhu, Sheng Ding
Life of this patent
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Abstract
The present invention provides for methods, compositions, and kits for producing an induced pluripotent stem cell from a non-pluripotent mammalian cell using a 3′-phosphoinositide-dependent kinase-1 (PDK1) activator or a compound that promotes glycolytic metabolism as well as other small molecules.
Claims11
A method of inducing a non-pluripotent mammalian cell into an induced pluripotent stem cell, the method comprising:
- (a) introducing into the non-pluripotent cell a polynucleotide encoding an Oct4 polypeptide;
- and
- (b) contacting the non-pluripotent cell with a small molecule 3′-phosphoinositide-dependent kinase-1 (PDK1) activator, a TGFβ receptor/ALK5 inhibitor, and a MEK inhibitor, thereby inducing the non-pluripotent mammalian cell into an induced pluripotent stem cell.
A mixture comprising:
- isolated mammalian cells, a small molecule PDK1 activator, and:
- (a) a TGFβ receptor/ALK5 inhibitor;
- (b) a MEK inhibitor;
- and
- (c) one or more vectors comprising a polynucleotide encoding one or more exogenous transcription factors selected from the group consisting of an Oct-4 polypeptide, a Klf-4 polypeptide, a c-myc polypeptide, and a Sox-2 polypeptide.
2 more elements
Dependencies are inferred from the claim text.