Crosslinked hyaluronan hydrogels for 3D cell culture
AssigneeUniversite De Rouen
InventorsJean-Pierre Vannier, Laurent David, Didier Lecerf, Virginie Dulong, Berenice Coquerel, Elise Demange
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Abstract
The present invention relates to a new method for treating or processing a cross-linked hyaluronan hydrogel prior to its use in applications involving three-dimensional cell culture. The invention also relates to cross-linked hyaluronan hydrogels prepared by such a method and to their use in 3D cell culture, stem cell/tissue engineering, drug discovery, toxicology testing, and broad cell biology applications.
Claims6
A method for obtaining a cross-linked hyaluronan hydrogel suitable for 3D cell culture, wherein the cross-linked hyaluronan hydrogel contains an organized, interconnected porous network with pores having an outer opening with an oblong or oval shape, is free of any tear or rip;
- and retains its shape following rehydration, said method comprising steps of:
- (a) lyophilizing the cross-linked hyaluronan hydrogel to obtain a lyophilized hyaluronan hydrogel or providing a lyophilized hyaluronan hydrogel;
- and
- (b) sterilizing the lyophilized hyaluronan hydrogel
- first by heating the hydrogel, and
- then by immersing the hydrogel in absolute ethanol at room temperature and subjecting the hydrogel to physical compression while the hydrogel is immersed in absolute ethanol, wherein the step of subjecting the hydrogel to physical compression eliminates any air trapped in the hydrogel.
Dependencies are inferred from the claim text.