Bacterial host strain
AssigneeUCB Pharma S.A.
InventorsMark Ellis, David Paul Humphreys
Life of this patent
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Abstract
A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest.
Claims20
A mutant E. coli cell strain selected from:
- MXE002 having genotype Δptr and deposited with the National Collection of Type Cultures, MPA, United Kingdom, under Accession number NCTC13445;
- MXE003 having genotype DegP S210A and deposited with the National Collection of Type Cultures, HPA, United Kingdom, under Accession number NCTC13446;
- or
- MXE006 having genotype Δptr, DegP S210A and deposited with the National Collection of Type Cultures, HPA, United Kingdom, under Accession number NCTC13449.
An isolated gram-negative bacterial cell comprising a mutated DegP gene, wherein the mutated DegP gene encodes a DegP protease that comprises a mutation at an amino acid selected from the group consisting of His105, Asp135, Ser210 and combinations thereof and said bacterial cell is isogenic to a wild-type bacterial cell except for the mutated DegP gene and, optionally, one or more of the following genetic alterations:
- a) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene;
- b) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene;
- and/or
- c) a polynucleotide encoding a protein of interest.
Dependencies are inferred from the claim text.