Antibodies and vaccines for use in therapeutic and diagnostic methods for α-synuclein-related disorders

A method of treating a neurodegenerative disorder with α-synuclein pathology in an individual, or delaying onset of a neurodegenerative disorder with α-synuclein pathology in an individual at risk of developing the disorder, wherein the disorder with α-synuclein pathology is selected from the group consisting of Parkinson's disease (PD), dementia with Lewy bodies (DLB), the Lewy body variant of Alzheimer's disease, and multiple system atrophy (MSA), comprising

• administering to the individual an antibody which is produced from a stabilized soluble α-synuclein protofibril and capable of binding a stabilized soluble α-synuclein protofibril, the stabilized soluble α-synuclein protofibril comprising a soluble α-synuclein protofibril modified with 4-hydroxy-2-nonenal, 4-oxo-2-nonenal, or a combination thereof and having a lower formation rate to a non-soluble aggregated form than a non-stabilized soluble protofibril of the α-synuclein, and the α-synuclein protofibril comprising wild type α-synuclein of SEQ ID NO:
• 1,
• wherein the antibody has been collected from a non-human animal to which stabilized soluble α-synuclein protofibril had been administered or wherein the antibody has been produced by hybridoma technology, phage display, ribosome display, mammalian cell display or bacterial display, and
• wherein the antibody is capable of binding wild type soluble α-synuclein protofibrils, has higher binding strength to soluble α-synuclein protofibril as compared with binding strength to α-synuclein monomers, and has higher binding strength to soluble α-synuclein protofibril as compared with binding strength to insoluble α-synuclein fibrils.