Peptides that inhibit angiotensin converting enzyme and peptides with antioxidant activity purified from ovotransferrin and methods of producing and using the same
AssigneeThe Governors of the University of Alberta
InventorsJianping Wu, Kaustav Majumder
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Abstract
Described herein are methods of identifying and releasing bioactive peptides from ovotransferrin The method involves (a) contacting ovotransferrin with a reducing agent, a sonication step, a high pressure processing step, a heating step, a fermentation step, or any combination thereof, and (b) contacting the ovotransferrin after step (a) with a hydrolytic enzyme to produce bioactive peptides that exhibit angiotensin converting enzyme inhibitory activity or antioxidant activity when compared to the parent protein. The bioactive peptides may be added to foodstuffs, a medication, or to any potable, ingestible, or edible compositions.
Claims19
A hydrolysate composition produced by the method comprising
- (a) contacting chicken egg ovotransferrin or a protein containing chicken egg ovotransferrin with a reducing agent, subjecting the chicken egg ovotransferrin or a protein containing chicken egg ovotransferrin to a sonication step, or a combination thereof;
- and
- (b) sequentially contacting the chicken egg ovotransferrin or a protein containing chicken ovotransferrin after step (a) with (1) thermolysin then pepsin or (2) pepsin then thermolysin to produce the hydrolysate composition, wherein the hydrolysate composition comprises at least one peptide selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2.
A peptide selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2.
Dependencies are inferred from the claim text.