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US 9,315,560 B2Granted
Purification of VWF for increased removal of non-lipid enveloped viruses
AssigneeBaxalta Incorporated
InventorsArtur Mitterer, Meinhard Hasslacher, Christa Mayer
Life of this patent
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Abstract
The present invention provides methods for purifying Von Willebrand factor (VWF) for increased removal of non-lipid enveloped viruses.
Claims9
A method for removing a non-lipid enveloped virus from a protein-containing solution comprising:
- applying the protein-containing solution to a cation exchange resin at a pH of 1.6 pH units or more above the isoelectric point of a protein in the protein-containing solution;
- and
- washing the cation exchange resin with a wash buffer to form an eluate, said wash buffer having a pH that is lower than the pH of the protein-containing solution applied to the cation exchange resin, wherein the protein has a molecular mass of at least about 150 kilodaltons, and whereby the non-lipid enveloped virus is removed from the protein-containing solution.
A method for removing a non-lipid enveloped virus from a VWF-containing solution comprising:
- applying the solution to a cation exchange resin, wherein the pH of the solution is 2.0 pH units or more above the isoelectric point of VWF;
- and
- washing the cation exchange resin with a wash buffer to form an eluate, said wash buffer having a pH that is equal to or lower than the pH of the solution applied to the cation exchange resin, and whereby the non-lipid enveloped virus is removed from the VWF-containing solution.
Dependencies are inferred from the claim text.