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US 9,315,559 B2Granted
Methods and compositions for generating an immune response by inducing CD40 and pattern recognition receptor adapters
AssigneeBaylor College of Medicine
InventorsDavid Spencer, Priyadharshini Narayanan
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Abstract
Provided are methods for activating an antigen-presenting cell and eliciting an immune response by inducing an inducible pattern recognition receptor adapter, or adapter fragment, and CD40 activity. Also provided are nucleic acid compositions comprising sequences coding for chimeric proteins that include an inducible CD40 peptide and an inducible pattern recognition receptor adapter or adapter fragment.
Claims18
A method for activating an antigen-presenting cell, which comprises:
- transfecting or transducing an antigen-presenting cell with a nucleic acid comprising a promoter operably linked to a polynucleotide that encodes a chimeric protein, wherein the chimeric protein comprises (i) a membrane targeting region, (ii) a multimeric ligand-binding region, (iii) a cytoplasmic CD40 polypeptide region lacking the extracellular domain, and (iv) a truncated MyD88 polypeptide lacking the TIR domain comprising the amino acid sequence SEQ ID NO:
- 6, wherein the chimeric protein is expressed in the antigen-presenting cell;
- and
- contacting the antigen-presenting cell with a non-protein multimeric ligand that binds to the ligand-binding region;
- whereby the antigen-presenting cell is activated.
2 more elements
A method for activating an antigen-presenting cell, which comprises:
- transfecting or transducing an antigen-presenting cell with a nucleic acid comprising a promoter operably linked to a polynucleotide that encodes a chimeric protein, wherein the chimeric protein comprises (i) a membrane targeting region, (ii) a multimeric ligand-binding region, and (iii) a region comprising a truncated MyD88 polypeptide lacking the TIR domain comprising the amino acid sequence SEQ ID NO:
- 6, wherein the truncated MyD88 polypeptide activates the NF-kappa B pathway, and wherein the chimeric protein is expressed in the antigen-presenting cell;
- and
- contacting the antigen-presenting cell with a non-protein multimeric ligand that binds to the ligand-binding region;
- whereby the antigen-presenting cell is activated.
2 more elements
Dependencies are inferred from the claim text.