US 9,315,558 B2 · Use of interleukin 10 mRNA transfected macrophages in anti-inflammatory therapies

Abstract

The present invention relates to the field of cell-based therapeutics. Specifically, the invention is concerned with a composition comprising a macrophage overexpressing interleukin 10 (IL-10) from transfected IL-10 encoding mRNA for use as a medicament. Moreover, a method for manufacturing a medicament for treating and/or preventing inflammation or a disease or disorder associated therewith comprising the steps of obtaining a macrophage from a sample of said subject, transfecting mRNA encoding IL-10 into said macrophage, and formulating said macrophage in a composition suitable for administration to the said subject, whereby the medicament is manufactured. Finally, a kit is provided for manufacturing such a medicament.

Claims7

Dependent claims

A method for treating and/or reducing local inflammation in a subject, comprising the steps of:

(a) obtaining a macrophage from the subject;
(b) transfecting mRNA encoding IL-10 into the macrophage, wherein said macrophage expresses IL-10;
(c) formulating the macrophage in a composition suitable for administration to the subject;
and
(d) administering the composition to the subject in a therapeutically effective dosage, thereby treating and/or reducing the local inflammation in the subject, wherein the local inflammation is associated with myocarditis, autoimmune disease, rheumatoid disease, rheumatoid arthritis, or multiple sclerosis.

2 more elements

Dependencies are inferred from the claim text.

Use of interleukin 10 mRNA transfected macrophages in anti-inflammatory therapies

Life of this patent

Use of interleukin 10 mRNA transfected macrophages in anti-inflammatory therapies

Life of this patent

Abstract

Claims7