Compositions and methods related to protein A (SpA) variants
AssigneeThe University of Chicago
InventorsOlaf Schneewind, Alice G. Cheng, Dominique M. Missiakas, Hwan Keun Kim
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Abstract
The present invention concerns methods and compositions for treating or preventing a bacterial infection, particularly infection by a Staphylococcus bacterium. The invention provides methods and compositions for stimulating an immune response against the bacteria. In certain embodiments, the methods and compositions involve a non-toxigenic Protein A (SpA) variant.
Claims14
A recombinant nucleic acid molecule comprising a nucleic acid sequence encoding a Staphylococcus aureus Protein A (SpA) D domain segment having (a) at least one amino acid substitution that disrupts Fc binding and (b) at least one amino acid substitution that disrupts VH3 binding and (c) an amino acid sequence that is at least 70% identical to the amino acid sequence of SEQ ID NO:
- 2, wherein the amino acid substitutions correspond to amino acids at position 9, 10, 36 and/or 37 of SEQ ID NO:
- 2;
- and wherein the substitutions at amino acids corresponding to positions 9 and 10 are with a glycine residue and the substitutions at amino acids corresponding to positions 36 and 37 are with a serine residue;
- or wherein the substitutions at amino acids corresponding to positions 9 and 10 are with a lysine residue and the substitutions at amino acids corresponding to positions 36 and 37 are with an alanine residue.
Dependencies are inferred from the claim text.