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US 9,315,536 B2Granted
Self-assembling oligonucleotides and methods
AssigneeCreatrone, Inc.
InventorsRoberto Crea, Guido Cappuccilli
Life of this patent
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Abstract
A composition comprising individual single-stranded oligonucleotides capable of self assembling to form a pair of complementary, substantially contiguous single strands of a double-stranded nucleic acid filament, and a method for forming such filaments are disclosed.
Claims6
A method of synthesizing a double-stranded nucleic acid filament, the method comprising:
- selecting a single species of an oligonucleotide including a first segment and a second segment wherein the first segment and the second segment:
- are of non-random sequence;
- have pre-determined lengths;
- have pre-adjusted ratios of A:T/G:C between 1% and 50%;
- forming by self-assembly under oligonucleotide hybridization conditions, a pair of substantially contiguous single strands of the double-stranded nucleic acid filament composed of multiple instances of the single species of the oligonucleotide;
- and
- terminating self-assembly of the double-stranded nucleic acid filament with chain terminator units, wherein the chain terminator units are composed of the nucleotides of the first segment or of the nucleotides of the second segment.
4 more elements
Dependencies are inferred from the claim text.