Non-N-glycosylated recombinant human annexin A2
AssigneeThe General Hospital Corporation
InventorZhirui Wang
Life of this patent
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Abstract
Methods for the expression and purification of non-N-glycosylated human Annexin A2 in yeast, e.g., in Pichia pastoris, purified Annexin A2 produced by those methods, and methods of using the Annexin A2.
Claims15
A non-N-glycosylated human annexin A2 comprising SEQ ID NO:1, wherein the amino acid at position 62 is alanine (A) or glycine (G);
- and/or the amino acid at position 64 is neither Threonine (T) nor Serine (S).
A method of treating a subject who has a vascular disorder associated with thrombus formation, the method comprising administering to the subject a therapeutically effective amount of the non-N-glycosylated human annexin A2 comprising SEQ ID NO:
- 1, wherein the amino acid at position 62 is alanine (A) or glycine (G);
- and/or the amino acid at position 64 is neither Threonine (T) nor Serine (S).
A method of producing a composition comprising a non-N-glycosylated human annexin A2, the method comprising:
- expressing a non-N-glycosylated human annexin A2 comprising SEQ ID NO:
- 1, wherein the amino acid at position 62 is alanine (A) or glycine (G);
- and/or the amino acid at position 64 is neither Threonine (T) nor Serine (S), in a methylotropic yeast;
- and
- substantially purifying the non-N-glycosylated human annexin A2,thereby producing the composition.
Dependencies are inferred from the claim text.