Cranberry xyloglucan oligosaccharide composition
AssigneesThe United States of America as represented by the Secretary of Agriculture, Ocean Spray Cranberries, Inc.
InventorsArland T. Hotchkiss, Alberto Nunez, Christina Khoo, Gary D. Strahan
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Abstract
A composition prepared from a cranberry hull enzyme-treated composition which reduces or inhibits the adhesion of microorganisms to cells having α-Gal-(1-4)-Gal terminal oligosaccharide receptors for adhesion.
Claims5
An anti-microbial composition, wherein said anti-microbial composition comprises a tablet or capsule having an effective amount of an anti-microbial adhesion inhibitory composition to inhibit the adhesions of bacteria to cells having α-Gal-(1-4)-β-Gal terminal oligosaccharide receptors for adhesion prepared by the method comprising the steps of:
- (a) providing cranberry hulls
- (b) treating said cranberry hulls with a pectinase in amounts sufficient to depectinize said hulls to form an enzyme treated composition,
- (c) removing debris from said enzyme treated composition using a method selected from the group consisting of decantation, centrifugation, and combinations thereof to form a first liquid,
- (d) spray drying said first liquid to form composition A1,
- (e) fractionating said composition A1 using C18 Flash Chromatography to produce composition A2
- (f) freeze drying said composition A2
- (g) purifying composition A2 using LH20 gel column chromatography to produce said anti-microbial adhesion inhibitory composition A6,
- (h) freeze drying said anti-microbial adhesion inhibitory composition A6 to produce a crystalline powder,
- (i) mixing said crystalline powder with a carrier to provide an anti-microbial adhesion inhibitory composition.
An anti-microbial composition, wherein said anti-microbial composition comprises a tablet or capsule having an effective amount of an anti-microbial adhesion inhibitory composition to inhibit the adhesions of bacteria to cells having α-Gal-(1-4)-β-Gal terminal oligosaccharide receptors for adhesion prepared comprising the steps of:
- (a) providing cranberry hulls
- (b) treating said cranberry hulls with a pectinase in amounts sufficient to depectinize said hulls to form an enzyme treated composition,
- (c) removing debris from said enzyme treated composition using a method selected from the group consisting of decantation, centrifugation, and combinations thereof to form a first liquid,
- (d) spray drying said first liquid to form composition A1,
- (e) fractionating said composition A1 using C18 Flash Chromatography to produce composition A2
- (f) freeze drying said composition A2
- (g) purifying fraction A2 LH20 using gel column chromatography to produce said anti-microbial adhesion inhibitory composition A6,
- (h) freeze drying said anti-microbial adhesion inhibitory composition A6 to produce a crystalline powder,
- (i) mixing said crystalline powder with a carrier to provide an anti-microbial adhesion inhibitory composition;
- wherein said anti-microbial adhesion inhibitory composition comprises arabino-xyloglucan with an SSG structure:
Dependencies are inferred from the claim text.