Treatment and prevention of mastitis

A method for treating or preventing clinical mastitis in a lactating dairy cow, comprising:

• injecting an effective amount of a cationic steroidal anti-microbial compound (CSA) formulation that is effective in treating or preventing clinical mastitis caused by one or more different microbes, including at least clinical mastitis caused by Prototheca to an intra-mammary organ of the lactating dairy cow, the CSA formulation containing at least one CSA compound of Formula I, or a pharmaceutically acceptable salt thereof:
• wherein
• rings A, B, C, and D are independently saturated;
• m, n, p, and q are independently 0 or 1;
• R1, R2, R4, R5, R6, R8, R9, R10, R11, R13, R14, R15, R16, and R17 are independently selected from the group consisting of hydrogen and substituted or unsubstituted (C1-C6) alkyl;
• R3, R7, R12, and R18 are independently selected from the group consisting of hydrogen, hydroxyl, substituted or unsubstituted (C1-C18) alkyl, substituted or unsubstituted (C1-C18) hydroxyalkyl, substituted or unsubstituted (C1-C18) alkyloxy-(C1-C18) alkyl, substituted or unsubstituted (C1-C18) alkylcarboxy-(C1-C18) alkyl, substituted or unsubstituted (C1-C18) alkylamino-(C1-C18) alkyl, substituted or unsubstituted (C1-C18) alkylamino-(C1-C18) alkylamino, substituted or unsubstituted (C1-C18) alkylamino-(C1-C18) alkylamino-(C1-C18) alkylamino, substituted or unsubstituted (C1-C18) aminoalkyl, substituted or unsubstituted aryl, substituted or unsubstituted arylamino-(C1-C18) alkyl, substituted or unsubstituted (C1-C18) haloalkyl, substituted or unsubstituted (C2-C6) alkenyl, substituted or unsubstituted (C2-C6) alkynyl, oxo, substituted or unsubstituted (C1-C18) aminoalkyloxy, substituted or unsubstituted (C1-C18) aminoalkyloxy-(C1-C18) alkyl, substituted or unsubstituted (C1-C18) aminoalkylcarboxy, substituted or unsubstituted (C1-C18) aminoalkylaminocarbonyl, substituted or unsubstituted (C1-C18) aminoalkylcarboxamido, substituted or unsubstituted di(C1-C18 alkyl) aminoalkyl, substituted or unsubstituted C-carboxy(C1-C18) alkyl, H2N—HC(Q5)-C(O)—O—, H2N—HC(Q5)-C(O)—N(H)—, substituted or unsubstituted (C1-C18) azidoalkyloxy, substituted or unsubstituted (C1-C18) cyanoalkyloxy, substituted or unsubstituted (C1-C18) guanidinoalkyloxy, substituted or unsubstituted (C1-C18) quaternary ammonium alkylcarboxy, and substituted or unsubstituted (C1-C18) guanidinoalkyl carboxy, where Q5 is a side chain of an amino acid;
• provided that at least two of R3, R7, R12 and R18 are independently selected from the group consisting of aminoalkyloxy, aminoalkylcarboxy, alkylaminoalkyl, and di(alkyl)aminoalkyl.

A method for treating or preventing clinical mastitis in a lactating dairy cow, comprising:

• injecting an effective amount of a cationic steroidal anti-microbial compound (CSA) formulation that is effective in treating or preventing clinical mastitis caused by one or more different microbes, including at least clinical mastitis caused by Prototheca, to an intra-mammary organ of the lactating dairy cow,
• wherein the CSA formulation comprises a compound having a sterol backbone of Formula (TB) or a pharmaceutically acceptable salt thereof:
• where,
• R3, R7, and R12 each include a cationic moiety attached to the sterol backbone via a hydrolysable linkage and R18 is selected from the group consisting of hydrogen, hydroxyl, substituted or unsubstituted (C1-C18) alkyl, substituted or unsubstituted (C1-C18) hydroxyalkyl, substituted or unsubstituted (C1-C18) alkyloxy-(C1-C18) alkyl, substituted or unsubstituted (C1-C18) alkylcarboxy-(C1-C18) alkyl, substituted or unsubstituted (C1-C18) alkylamino-(C1-C18) alkyl, substituted or unsubstituted (C1-C18) alkylamino-(C1-C18) alkylamino, substituted or unsubstituted (C1-C18) alkylamino-(C1-C18) alkylamino-(C1-C18) alkylamino, substituted or unsubstituted (C1-C18) aminoalkyl, substituted or unsubstituted aryl, substituted or unsubstituted arylamino-(C1-C18) alkyl, substituted or unsubstituted (C1-C18) haloalkyl, substituted or unsubstituted (C2-C6) alkenyl, substituted or unsubstituted (C2-C6) alkynyl, oxo, substituted or unsubstituted (C1-C18) aminoalkyloxy, substituted or unsubstituted (C1-C18) aminoalkyloxy-(C1-C18) alkyl, substituted or unsubstituted (C1-C18) aminoalkylcarboxy, substituted or unsubstituted (C1-C18) aminoalkylaminocarbonyl, substituted or unsubstituted (C1-C18) aminoalkylcarboxamido, substituted or unsubstituted di(C1-C18 alkyl) aminoalkyl, substituted or unsubstituted C-carboxy(C1-C18) alkyl, H2N—HC(Q5)-C(O)—O—, H2N—HC(Q5)-C(O)—N(H)—, substituted or unsubstituted (C1-C18) azidoalkyloxy, substituted or unsubstituted (C1-C18) cyanoalkyloxy, substituted or unsubstituted (C1-C18) guanidinoalkyloxy, substituted or unsubstituted (C1-C18) quaternary ammonium alkylcarboxy, and substituted or unsubstituted (C1-C18) guanidinoalkyl carboxy, where Q5 is a side chain of an amino acid.