Method for providing images of a tissue section

A method of differentiating areas in a series of N primary digital images of a tissue section wherein N is an integer >1, thereby creating a new image, said method comprising:

• a) providing a series of N primary digital images comprising undetermined marker areas, wherein an image In+1 comprises at least the same amount of undetermined marker areas as a primary digital image In for 2<n<N, wherein n is an integer;
• b) evaluating every primary digital image In for 1<n<N according to predetermined selection criteria and defining image marker areas as undetermined marker areas fulfilling the predetermined selection criteria, thereby obtaining a series of N secondary digital images, and storing information about any such image marker area in or in connection/association with a corresponding secondary digital image in the obtained series of secondary digital images;
• c) providing a new image Inew;
• d) for every n for 2<n<N of the series of secondary digital images obtained in operation b), inserting new image marker areas in the new image Inew, said new image marker areas having the same shape and location as image marker areas present in image In but not in image In−1, said new image marker areas being identifiable in Inew by a unique feature;
• e) inserting new image marker areas in the new image Inew, said new image marker areas having the same shape and location as image marker areas present in image I1, and said image marker areas being identifiable in Inew, by a unique feature.