Feline bitter taste receptors and methods
AssigneeApplied Food Biotechnology, Inc.
InventorsMichelle M. Sandau, Nancy E. Rawson
Life of this patent
Estimating the term…
Abstract
A family of novel feline bitter taste receptors, referred to as feline TAS2R (fTAS2R), are disclosed herein. Isolated polynucleotides encoding the novel feline bitter taste receptors and chimeric polypeptides are also disclosed, as are expression vectors and host cells for expression of the novel feline bitter taste receptors. Methods of identifying compounds that bind to the novel feline bitter taste receptors and modulate their activity are disclosed.
Claims20
A polypeptide comprising
- the amino acid sequence of SEQ ID NO:2, or
- an amino acid sequence having at least 95% homology to the sequence of SEQ ID NO:2;
- wherein the polypeptide is covalently bound to a label, a solid support, or a lipid monolayer.
A chimeric polypeptide comprising at least one extracellular domain of SEQ ID NO:2 covalently linked to a heterologous polypeptide sequence, wherein the extracellular domain comprises amino acids 68-84;
- 146-179;
- or 249-257 of SEQ ID NO:2.
A chimeric polypeptide comprising at least one intracellular domain of SEQ ID NO:2 covalently linked to a heterologous polypeptide sequence, wherein the intracellular domain comprises 23-46;
- 106-124;
- 201-227;
- or 279-298 of SEQ ID NO:2.
A chimeric polypeptide comprising at least one transmembrane domain of SEQ ID NO:2 covalently linked to a heterologous polypeptide sequence, wherein the transmembrane domain comprises amino acids 2-22, 47-67, 85-105, 125-145, 180-200, 228-248, or 258-278 of SEQ ID NO:2.
Dependencies are inferred from the claim text.