Luminophore-labeled molecules coupled with particles for microarray-based assays

A method for detecting a target molecule using a microarray, which method comprises:

• a) labeling the target molecule with a luminophore;
• b) coupling the target molecule to a particle;
• c) binding the target molecule that is labeled with the luminophore and coupled to the particle, to a probe molecule immobilized on the microarray;
• and
• d) detecting the interaction between the target molecule and the probe molecule,
• wherein the target molecule is a polynucleotide,
• wherein the method further comprises before the detecting step a step of amplifying the target polynucleotide, and
• wherein the target polynucleotide is amplified using at least two allele-specific primers and their corresponding common primer as set forth in Table 2.

A method for detecting a target molecule using a microarray, which method comprises:

• a) labeling the target molecule with a luminophore;
• b) coupling the target molecule to a particle;
• c) binding the target molecule that is labeled with the luminophore and coupled to the particle, to a probe molecule immobilized on the microarray;
• and
• d) detecting the interaction between the target molecule and the probe molecule,
• wherein the target molecule is selected from the group consisting of a polynucleotide, a polypeptide, an antibody, a small molecule compound, a peptide and a carbohydrate,
• wherein the microarray comprises a universal tag array, and
• wherein the universal tag array comprises at least two of the Tag sequences as set forth in Table 1.