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US 9,310,372 B2Granted
Method for the selection of specific affinity binders by homogeneous noncompetitive assay
AssigneeDynamic Affinity Reagents, LLC
InventorWilliam Patterson
Life of this patent
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Abstract
The invention generally relates to the field of immunochemistry including antibody therapy, diagnostics, and basic research and specifically relates to the area of selecting affinity molecules such as natural antibodies, including artificial antibodies, antibody mimics, and aptamers. The invention relates particularly to a method of selecting affinity molecules using a homogeneous noncompetitive assay in a high throughput process.
Claims12
A method for detecting affinity molecule complexes in a homogeneous non-competitive assay, said method comprising:
- a) forming affinity molecule complexes with i) a target molecule, ii) a first molecule, iii) a detection molecule that is a first member of a complementary pair, wherein the detection molecule is fused to the first molecule;
- and iv) a second molecule comprising a candidate affinity molecule fused to a complementary detection molecule that is a second member of the complementary pair;
- wherein the binding affinity of the candidate affinity molecule for the target is not known, and wherein said detection molecule and said complementary detection molecule generate a measurable signal upon binding of said candidate affinity molecules to said target;
- and b) detecting said measurable signal.
1 more element
Dependencies are inferred from the claim text.