High affinity monoclonal antibodies for detection of Shiga toxin 2 (STX2)
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsXiaohua He, Larry H. Stanker, Luisa W. Cheng
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Abstract
High affinity monoclonal antibodies against Stx2 and hybridomas that produce such antibodies are described. The antibodies may be used in a kit for detecting Stx2 and variants thereof in a sample as well as neutralization of Shiga toxin in vivo.
Claims6
A monoclonal antibody produced by a continuous hybridoma cell line selected from the group consisting of deposit accession number PTA-13614 and PTA-13615.
A method for detecting Shiga toxin 2 and variants thereof comprising (1) incubating a sample with the monoclonal antibody produced by a continuous hybridoma selected from the group consisting of deposit accession number PTA-13614 and PTA-13615, and mixtures thereof;
- and (2) detecting the antibody-Shiga toxin 2 complex wherein the presence or absence of the complex indicates the presence or absence of Shiga toxin 2 in the sample.
A kit for detecting Shiga toxin 2 in a sample, said kit comprising;
- (1) a container comprising a monoclonal antibody produced by a continuous hybridoma selected from the group consisting of deposit accession number PTA-13614 and PTA-13615, and mixtures thereof:
- and (2) instructions for using the antibody for the purpose of binding to Shiga toxin 2 to form, an immunological complex and detecting the formation of the immunological complex suck that presence or absence of immunological complex correlates with presence or absence of Shiga toxin 2 in said sample.
Dependencies are inferred from the claim text.