Multifunctional encoded particles for high-throughput analysis
AssigneeMassachusetts Institute of Technology
InventorsPatrick S. Doyle, Daniel C. Pregibon
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Abstract
Method for making multifunctional particles. The method includes flowing a first monomer stream loaded with a fluorescent entity along a microfluidic channel and flowing a second monomer stream loaded with a probe adjacent to the first monomer stream along the microfluidic channel. The monomer streams are polymerized to synthesize particles having a fluorescent, graphically encoded region and a probe-loaded region.
Claims18
A polymeric particle comprising at least one encoded region and at least one probe-loaded region, the at least one encoded region being a distinct region from the at least one probe-loaded region, wherein the polymeric particle is a PEG hydrogel particle synthesized from a first and second monomer streams;
- wherein the encoded region is synthesized from the first monomer stream, and the probe-loaded region is synthesized from the second monomer stream loaded with at least one oligonucleotide probe at a concentration of between 50 μM and 150 μM, such that the probe is embedded within the particle and further wherein the particle is greater than about 100 μm up to about 450 μm in at least one dimension.
Dependencies are inferred from the claim text.