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US 9,310,359 B2Granted
Analyte quantification using flow induced dispersion analysis
AssigneeFida-Tech Aps
InventorsHenrik Jensen, Jesper Ostergaard
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Abstract
A method for quantitative characterization of non-covalent interactions and analyte quantification in nanoliter samples is described. The procedure is based on Flow Induced Dispersion Analysis (FIDA), of which the only system requirements is a narrow tube, capillary or channel equipped with a detector. The technique can be implemented using standard equipment such as High Performance Liquid Chromatography (HPLC), Flow Injection Analysis (FIA) or Capillary Electrophoresis (CE).
Claims18
A method for measuring the concentration of an analyte in a sample, comprising:
- (a) for each of a plurality of concentrations of the analyte:
- (i) flowing the analyte in the presence of a concentration of a ligand, in solution under pressure through a narrow tube, capillary, or channel, wherein the ligand is an affinity probe of the analyte and forms a complex with the analyte, wherein the flowing satisfies conditions of Taylor dispersion, and wherein at least one of the plurality of concentrations of the analyte is zero;
- (ii) measuring the flow induced dispersion of the ligand by detecting a UV or fluorescence signal generated by the ligand;
- and
- (iii) calculating a variance of a peak of the signal;
- (b) generating a curve of the peak variances from step (a) by plotting the peak variances against their respective analyte concentrations, thereby generating a standard curve;
- (c) flowing the analyte from the sample in the presence of the concentration of the ligand under pressure through the narrow tube, capillary, or channel, wherein the flowing satisfies conditions of Taylor dispersion;
- (d) measuring the flow induced dispersion of the ligand by detecting a UV or fluorescence signal generated by the ligand of step (c) with the detector;
- (e) calculating a variance of a peak of the signal from step (d);
- and
- (f) comparing the variance from step (e) to the standard curve,
- wherein the closest match between the variance from step (e) and a point on the standard curve determines the concentration of the analyte in the sample.
9 more elements
A method for measuring the concentration of a first analyte in a sample, comprising:
- (a) for each of a plurality of concentrations of the first analyte:
- (i) flowing the first analyte in the presence of a concentration of a ligand and a concentration of a second analyte, in solution under pressure through a narrow tube, capillary, or channel, wherein the ligand is an affinity probe of the first analyte and the second analyte and forms a complex with the first analyte and alternatively forms a complex with the second analyte, wherein the second analyte is distinguishable from the second analyte, and wherein the flowing satisfies conditions of Taylor dispersion;
- (ii) measuring the flow induced dispersion of the second analyte by detecting a UV or fluorescence signal generated by the second analyte;
- and
- (iii) calculating a variance of a peak of the signal;
- (b) generating a curve of the peak variances from step (a) by plotting the peak variances against their respective first analyte concentrations, thereby generating a standard curve;
- (c) flowing the first analyte from the sample in the presence of the concentration of the ligand and of the concentration of the second analyte under pressure through the narrow tube, capillary, or channel, wherein the flowing satisfies conditions of Taylor dispersion;
- (d) measuring the flow induced dispersion of the second analyte by detecting a UV or fluorescence signal generated by the second analyte of step (c) with the detector;
- (e) calculating a variance of a peak of the signal from step (d);
- and
- (f) comparing the variance from step (e) to the standard curve,
- wherein the closest match between the variance from step (e) and a point on the standard curve determines the concentration of the first analyte in the sample.
9 more elements
Dependencies are inferred from the claim text.