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US 9,310,353 B2Granted
Method for evaluating bioavailable zinc
AssigneeThe Procter & Gamble Company
InventorsJohn Christian Haught, Koti Tatachar Sreekrishna, Yakang Lin
Life of this patent
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Abstract
A method to detect bioavailable metal ions from an oral care composition and a method to select for compounds that inhibit the uptake of metal ions into cells.
Claims17
Method for the detection of intracellular Zn uptake comprising:
- providing TR146 cells;
- contacting Zn with the TR146 cells;
- adding a Zn-indicator having a Zn-binding group and a signaling moiety, wherein the signaling moiety comprises a detectable chemical moiety;
- measuring the amount of Zn uptake within the TR146 cells, by detecting the signaling chemical moiety of the Zn-indicator within the TR146 cells.
1 more element
Method for screening compounds that reduce intracellular Zn uptake comprising:
- providing TR146 cells;
- contacting Zn with the TR146 cells;
- adding a Zn-indicator having a Zn-binding group and a signaling moiety, wherein the signaling moiety comprises a detectable chemical moiety;
- measuring the amount of Zn uptake within the TR146 cells, by detecting the signaling chemical moiety of the Zn-indicator within the TR146 cells;
- providing TR146 cells;
- adding a potential Zn uptake blocker to the TR146 cells;
- adding Zn to the TR146 cells;
- adding a Zn-indicator having a Zn-binding group and a signaling moiety, wherein the signaling moiety comprises a detectable chemical moiety;
- measuring the amount of Zn uptake within the TR146 cells, by detecting the signaling chemical moiety of the Zn-indicator within the TR146 cells, to determine if the potential Zn uptake blocker reduces Zn uptake within the TR146 cells.
6 more elements
Method for determining the astringency of a Zn ion containing oral care composition comprising:
- providing TR146 cells;
- contacting a zinc ion containing oral care composition with the TR146 cells;
- adding a Zn-indicator having a Zn-binding group and a signaling moiety, wherein the signaling moiety comprises a detectable chemical moiety;
- measuring the amount of Zn uptake within the TR146 cells, by detecting the signaling chemical moiety of the Zn-indicator within the TR146 cells.
1 more element
Dependencies are inferred from the claim text.