Systems and methods of detecting and demonstrating hair damage via evaluation of protein fragments

A method for demonstrating bleach or UV hair damage, the method comprising:

• a) extracting the labile proteins using a suitable solvent from a hair sample to provide a protein fragment sample;
• b) analyzing the protein fragments sample with MALDI-MS;
• resulting in protein fragment results;
• c) identifying the hair damage by comparing the protein fragments results to a list of marker protein fragments for particular damage;
• wherein step a) comprises removing the labile protein from the hair sample via contacting the hair sample with the suitable solvent, wherein the suitable solvent is an aqueous solution without the addition of any reduction or extraction agents;
• wherein the MALDI-MS is performed without the enzymatic digestion of proteins;
• and
• wherein the list of marker protein fragments include a +28 and +71 modification to the N-terminus of the protein fragment having the structure:
• for identifying bleach damage, or a protein marker with m/z 1278 for identifying UV damage.