HILIC/anion-exchange/cation-exchange multimodal media
AssigneeDionex Corporation
InventorsXiaodong Liu, Christopher A. Pohl, Jinhua Chen
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Abstract
The present invention provides an agglomerated multimodal chromatographic medium. the medium of the invention includes groups active in anion exchange, cation exchange and hydrophilic interaction chromatographic modalities. The invention provides methods of making these media and using them in separations of analytes. Also provided are separations devices incorporating the medium and systems incorporating these separations devices.
Claims25
An agglomerated multimodal chromatographic medium with hydrophilic interaction, cation exchange and anion exchange properties, said medium being suitable for use as chromatographic packing and comprising:
- (a) component A comprising substrate particles functionalized with an ion exchange ligand comprising a negatively charged carboxylate ion exchange group, and a hydrophilic ligand comprising a neutral polar group, wherein neither said ion exchange ligand nor said hydrophilic ligand contain more than 5 non-aromatic carbon atoms in series and each of said ion exchange ligand and said hydrophilic ligand is bound to said substrate, and at least one of said ion exchange ligand, said hydrophilic ligand and a combination thereof has the formula:
- in which u and v are integers independently selected from the integers 1, 2, 3, 4, and 5;
- and
- (b) component B comprising particles of a second substrate having positively charged external surfaces,
- wherein component A and component B are bound by electrostatic forces between said charged ion exchange ligand of component A and said charged external surfaces component B forming, in composite, an agglomerated medium of ion exchange particles.
An agglomerated multimodal chromatographic medium with hydrophilic interaction, cation exchange and anion exchange properties, said medium being suitable for use as chromatographic packing and comprising:
- (a) component A comprising substrate particles functionalized with an ion exchange ligand comprising a negatively charged carboxylate ion exchange group, and a hydrophilic ligand comprising a neutral polar group, in which at least one of said ion exchange ligand, said hydrophilic ligand and a combination thereof-has the formula:
- in which u and v are integers independently selected from the integers 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10;
- and
- (b) component B comprising particles of a second substrate having positively charged external surfaces,
- wherein component A and component B are bound by electrostatic forces between said charged ion exchange ligand of component A and said charged external surfaces component B forming, in composite, an agglomerated medium of ion exchange particles.
Dependencies are inferred from the claim text.