Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue

At least one non-transitory computer readable storage media comprising computer-executable instructions for:

• receiving a first image and a second image associated with a common portion of a specimen section, wherein the first image is a refractive dark field image and the second image is a fluorescence image;
• combining the refractive dark field image and the fluorescence image and generating a refractive dark field and fluorescence combined image, wherein before the refractive dark field image is combined with the fluorescence image, the refractive dark field image is processed based on a color lookup table associated with an eosin stain and the fluorescence image is processed based on color lookup table associated with a hematoxylin stain, and wherein the refractive dark field and fluorescence combined image is based on the processed first and second images;
• inverting the refractive dark field and fluorescence combined image, and generating a pseudo-color brightfield hematoxylin and eosin image based on the processed first image and the processed second image.

An image processor, comprising:

• image inputs configured that receives a first image and a second image, wherein the first image is a refractive dark field image and the second image is a fluorescent image;
• a color lookup table input that receives, a first color lookup table associated with an eosin stain and a second color lookup table associated with a hematoxylin stain;
• an image combiner that processes the refractive dark field image based on the first color lookup table associated with an eosin stain and that processes the fluorescent image based on the second color lookup table associated with the eosin stain and produces a pseudo-color refractive dark field image and a pseudo-color fluorescent image, and combines the pseudo-color refractive dark field image with the pseudo-color fluorescent image and generates a combined refractive dark field and fluorescent image, and produces a brightfield image rendering based on the combined refractive dark field and fluorescent image after inverting the combined refractive dark field and fluorescent image.